{"id":5,"date":"2012-10-25T22:04:15","date_gmt":"2012-10-25T22:04:15","guid":{"rendered":"http:\/\/pages.charlotte.edu\/template-faculty01\/?page_id=5"},"modified":"2013-04-08T19:09:13","modified_gmt":"2013-04-08T19:09:13","slug":"home","status":"publish","type":"page","link":"https:\/\/pages.charlotte.edu\/brian-cooper\/","title":{"rendered":"Home"},"content":{"rendered":"<p><strong><a href=\"http:\/\/pages.charlotte.edu\/brian-cooper\/wp-content\/uploads\/sites\/229\/2012\/10\/bcooper.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-full wp-image-10\" title=\"bcooper\" alt=\"\" src=\"http:\/\/pages.charlotte.edu\/brian-cooper\/wp-content\/uploads\/sites\/229\/2012\/10\/bcooper.jpg\" width=\"110\" height=\"180\" \/><\/a>B.S.<\/strong>\u00a0: Purdue University, 1989<br \/>\n<strong>Ph.D.<\/strong> : University of Arizona, 1994<br \/>\n<strong>Post-doc<\/strong>\u00a0: (NIH Fellow) Iowa State University, 1994\u20131997<\/p>\n<p>ORAU Junior Faculty Enhancement Award, 1999\u20132000<br \/>\nNSF Faculty Early Career Development (CAREER) Award, 2000\u20132004<\/p>\n<p><strong>Courses Taught:<\/strong><\/p>\n<p>Principles of Chemistry (<a href=\"http:\/\/bunsen.uncc.edu\/courses\/1251\/\">CHEM 1251)<\/a><br \/>\nPrinciples of Chemistry\u00a0<a href=\"http:\/\/bunsen.uncc.edu\/courses\/1252\/\">(CHEM 1252)<\/a><br \/>\nQuantitative Analysis (<a href=\"http:\/\/bunsen.uncc.edu\/faculty\/cooper\/3111\/\">CHEM 3111<\/a>\u00a0)<br \/>\nInstrumental Analysis and Lab (<a href=\"http:\/\/bunsen.uncc.edu\/courses\/4111\/\">CHEM 4111\/4111L<\/a>\u00a0)<br \/>\nProtein Analysis by MALDI-MS (CHEM 4090\/5090)<br \/>\nMass Spectrometry (CHEM 6115)<\/p>\n<p><strong>Research Focus:<\/strong><\/p>\n<p>My research group primarily uses capillary electrophoresis (CE) to analyze and characterize proteins. Capillary electrophoretic separations of protein &#8220;charge ladders&#8221; (otherwise pure proteins with intrinsic or induced charge heterogeneity) allow us to estimate the net charge and hydrodynamic radius of proteins in solution. We also study ligand binding to proteins using &#8220;affinity capillary electrophoresis&#8221; (ACE), which exploits the accompanying change in protein electrophoretic mobility. Combining charge ladders and ACE allows us to characterize overall conformational changes caused by ligand binding. And with laser-induced fluorescence (LIF) detection, we can study the conformational behavior of fluorescently labeled proteins under simulated intracellular conditions\u2014especially in the presence of high concentrations of other macromolecules.<\/p>\n<p>We also have an active collaboration with a group in the Department of Bioinformatics and Genomics. We are using a variant of ACE called &#8220;CEMSA&#8221; (capillary electrophoretic mobility shift assay) to detect binding of transcription factors (TFs) to synthetic, fluorescently labeled DNA probes. We use this technique to experimentally validate predicted TF binding site sequences. After screening by CEMSA, we can identify affinity-purified TFs using mass spectrometry.<br \/>\n<strong>Overview Academic\/Research Interest Areas:<\/strong><\/p>\n<p>Bioanalytical Chemistry<\/p>\n<p>Protein analysis by: capillary\/channel electrophoresis<\/p>\n<p>Ultrasensitive fluorescence detection and imaging<\/p>\n<p>Electrospray and MALDI mass spectrometry<br \/>\n<strong>What he brings to the industry:<\/strong><\/p>\n<p>Novel methods or applications for capillary electrophoresis<\/p>\n<p>New methods for electrospray and MALDI mass spectrometry, capillary liquid chromatography, and ultrasensitive fluorescence detection.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>B.S.\u00a0: Purdue University, 1989 Ph.D. : University of Arizona, 1994 Post-doc\u00a0: (NIH Fellow) Iowa State University, 1994\u20131997 ORAU Junior Faculty Enhancement Award, 1999\u20132000 NSF Faculty Early Career Development (CAREER) Award, 2000\u20132004 Courses Taught: Principles of Chemistry (CHEM 1251) Principles of Chemistry\u00a0(CHEM 1252) Quantitative Analysis (CHEM 3111\u00a0) Instrumental Analysis and Lab (CHEM 4111\/4111L\u00a0) Protein Analysis by [&hellip;]<\/p>\n","protected":false},"author":27,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-5","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P2X0RR-5","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/pages.charlotte.edu\/brian-cooper\/wp-json\/wp\/v2\/pages\/5","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pages.charlotte.edu\/brian-cooper\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pages.charlotte.edu\/brian-cooper\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pages.charlotte.edu\/brian-cooper\/wp-json\/wp\/v2\/users\/27"}],"replies":[{"embeddable":true,"href":"https:\/\/pages.charlotte.edu\/brian-cooper\/wp-json\/wp\/v2\/comments?post=5"}],"version-history":[{"count":8,"href":"https:\/\/pages.charlotte.edu\/brian-cooper\/wp-json\/wp\/v2\/pages\/5\/revisions"}],"predecessor-version":[{"id":19,"href":"https:\/\/pages.charlotte.edu\/brian-cooper\/wp-json\/wp\/v2\/pages\/5\/revisions\/19"}],"wp:attachment":[{"href":"https:\/\/pages.charlotte.edu\/brian-cooper\/wp-json\/wp\/v2\/media?parent=5"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}