{"id":235,"date":"2010-06-28T12:07:49","date_gmt":"2010-06-28T16:07:49","guid":{"rendered":"http:\/\/chemistry.uncc.edu\/people\/jkrueger\/?p=235"},"modified":"2012-11-15T19:47:21","modified_gmt":"2012-11-15T19:47:21","slug":"06252010","status":"publish","type":"post","link":"https:\/\/pages.charlotte.edu\/joanna-krueger\/blog\/2010\/06\/28\/06252010\/","title":{"rendered":"06\/25\/2010"},"content":{"rendered":"<p>Jon Trullinger has measured a depolymerization rate for f-actin after addition of 5 wt% ethylammonium nitrate (EAN) using the Fluo-STAR Galaxy Plate reader (Ex @ 355 nm and Em @ 405nm)<\/p>\n<p><a href=\"http:\/\/pages.charlotte.edu\/joanna-krueger\/wp-content\/uploads\/sites\/151\/2010\/06\/ActinDepolybyEAN_fluorstar1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-medium wp-image-237\" src=\"http:\/\/pages.charlotte.edu\/joanna-krueger\/wp-content\/uploads\/sites\/151\/2010\/06\/ActinDepolybyEAN_fluorstar1-300x252.jpg\" alt=\"\" width=\"300\" height=\"252\" srcset=\"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-content\/uploads\/sites\/151\/2010\/06\/ActinDepolybyEAN_fluorstar1-300x252.jpg 300w, https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-content\/uploads\/sites\/151\/2010\/06\/ActinDepolybyEAN_fluorstar1-1024x860.jpg 1024w, https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-content\/uploads\/sites\/151\/2010\/06\/ActinDepolybyEAN_fluorstar1.jpg 1052w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>The decrease in fluorescence intensity of pyrene-labeled actin measured as a function of time post-mixing with EAN\u00a0 shows that EAN has the ability to depolymerize actin.\u00a0 After a 300 sec equilibration time, 10 \u03bcL 50 wt% EAN was delivered, via an in-line syringe pump, to a well containing 100 \u03bcL 38 \u03bcg\/ml pyrene-labeled F-actin.\u00a0 Depolymerization is observed as a decrease in fluorescence signal.\u00a0 Data were normalized to the maximum intensity measured prior to depolymerization for each sample. Measurements were done\u00a0 in triplicate.\u00a0 The average normalized intensity values with their standard deviation are shown.\u00a0 OriginPro v7.5 was used to determine the exponential fit parameters (a, b, k)\u00a0 as well as the initial rate of depolymerization.<\/p>\n<p>y = a+be^(-kx)<\/p>\n<p>a=0.02\u00a0\u00a0\u00a0 b=1.51\u00a0\u00a0\u00a0 k=0.0043<\/p>\n<p>Rate = -0.015\/sec<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Jon Trullinger has measured a depolymerization rate for f-actin after addition of 5 wt% ethylammonium nitrate (EAN) using the Fluo-STAR Galaxy Plate reader (Ex @ 355 nm and Em @ 405nm) The decrease in fluorescence intensity of pyrene-labeled actin measured as a function of time post-mixing with EAN\u00a0 shows that EAN has the ability to [&hellip;]<\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-235","post","type-post","status-publish","format-standard","hentry","category-dotw2010"],"_links":{"self":[{"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/posts\/235","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/comments?post=235"}],"version-history":[{"count":1,"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/posts\/235\/revisions"}],"predecessor-version":[{"id":289,"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/posts\/235\/revisions\/289"}],"wp:attachment":[{"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/media?parent=235"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/categories?post=235"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pages.charlotte.edu\/joanna-krueger\/wp-json\/wp\/v2\/tags?post=235"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}