Faculty Connections
Faculty Connections

About this site

Faculty Connections is an aggregation of UNC Charlotte faculty profiles.

Full-time faculty who want to update their profile information, see:
Connection Update

UNC Charlotte faculty and staff can log in with their NinerNET user accounts.
Log In

Colleges & Departments

  • ►Belk College of Business
    • ►Business Info Systems/Operations
    • ►Economics
    • ►Finance
    • ►Management
    • ►Marketing
    • ►Turner School of Accountancy
  • ►College of Arts + Architecture
    • ►Art & Art History
    • ►Dance
    • ►Music
    • ►Performing Arts Services
    • ►School of Architecture
    • ►Theatre
  • ►College of Computing & Informatics
    • ►Bioinformatics and Genomics
    • ►Bioinformatics Research Center
    • ►Computer Science
    • ►Software & Information Systems
  • ►College of Education
    • ►Counseling
    • ►Ctr For STEM Education
    • ►Educational Leadership
    • ►Middle Grades Secondary & K-12
    • ►Reading & Elementary ED
    • ►School & Community Partnerships
    • ►Special Ed & Child Dev
  • ►College of Health & Human Services
    • ►Appl Phys Hlth & Clin Sci
    • ►Kinesiology
    • ►Public Health Sciences
    • ►School of Nursing
    • ►School of Social Work
  • ►College of Liberal Arts & Sciences
    • ►Africana Studies
    • ►Anthropology
    • ►Biological Sciences
    • ►Chemistry
    • ►Communication Studies
    • ►Criminal Justice and Criminology
    • ►English
    • ►Geography and Earth Sciences
    • ►Global Studies
    • ►History
    • ►Languages and Culture Studies
    • ►Mathematics and Statistics
    • ►Philosophy
    • ►Physics and Optical Science
    • ►Political Science and Public Administration
    • ►Psychological Science
    • ►Religious Studies
    • ►Sociology
    • ►Writing, Rhetoric, and Digital Studies
  • ▼Lee College of Engineering
    • ►Civil and Environmental Engr
    • ►Electrical & Computer Engineering
    • ►Engineering Tech & Constr Mgmt
    • ►EPIC
    • ▼Mech Engineering & Engineering Sci
      • Ahmed El-Ghannam
      • Alexandra Stefanescu
      • Alireza Tabarraei
      • Amirhossein Ghasemi
      • Anthony Bombik
      • Artur Wolek
      • Arun Vishnu Suresh Babu
      • Bamdad Lessani
      • Brigid Mullany
      • Charles Jenckes
      • Charles Lee
      • Christoph Kossack
      • Christopher Evans
      • Edward Morse
      • Erina Joyee
      • Gloria Elliott
      • HaiTao Zhang
      • Harischandra Cherukuri
      • Hongbing Fang
      • In Hong Yang
      • Jeffrey Raquet
      • Jerry Dahlberg
      • John McAlpine
      • Jonathan Beaman
      • Joseph Dalton
      • Joshua Tarbutton
      • Jun Xu
      • Kamia Smith
      • Kevin Lawton
      • Kevin Smith
      • Konstantinos Falaggis
      • Lin Ma
      • Martin Hodgins
      • Matthew Davies
      • Mesbah Uddin
      • Naiquan Zheng
      • Nenad Sarunac
      • Peter Tkacik
      • Praveen Ramaprabhu
      • Qiuming Wei
      • Regina Vrikkis
      • Russell Keanini
      • Scott Kelly
      • Steven Patterson
      • Steven Schmid
      • Stuart Smith
      • Terence Fagan
      • Thomas Koch
      • Ting Xu
      • Tony Schmitz
      • Tyler Stover
      • Youxing Chen
    • ►Student Dev & Success
    • ►Systems Engin & Engin Management
  • ►School of Data Science (SDS)

Programs, Centers & Initiatives

  • ►Center for Applied Geographic Information Science
  • ►Metropolitan Studies
    • ►Urban Institute

Top Links

  • performing arts
  • art
  • visual arts
  • architecture
  • diversity
  • music
  • gender
  • culture
  • theatre
  • design
  • education
  • race
  • arts and community
  • arts education
  • dance
  • environment
  • robotics
  • health disparities
  • art history
  • machine learning
  • statistics
  • sexuality
  • rhetoric
  • composition
  • health
  • computer science
  • sustainability
  • ecology
  • evolution
  • vulnerable populations
  • literacy
  • mental health
  • urban design
  • collaboration
  • politics
  • popular culture
  • biostatistics
  • mathematical physics
  • teacher education
  • artificial intelligence
  • music education
  • ethics
  • social media
  • molecular biology
  • orchestra

Full-Text Search

Charles Lee

Mech Engineering & Engineering Sci
biodevices
biomathematical modeling
biomedical engineering
heat transfer
mass transfer
Related People
Nenad Sarunac
Didier Dreau

Charles Y. Lee, Ph.D. Webpage

Bioheat and Mass Transfer Laboratory:

Research: Currently we are working in a number of research areas related to Biomedical Engineering

 

Hypothermic machine perfusion preservation of livers for transplantation:

With over 15,000 patients waiting for liver transplantation and over 1100 deaths a year as a result of organ shortages, there is a need to improve preservation of livers and new ways to expand the donor pool. Hypothermic machine perfusion (HMP) has the potential to provide better quality livers for transplant and reclaim marginal organs that currently are unusable. Currently, HMP has not improved long-term storage (>24 hrs) of livers and our group is investigating the cause of damage. Three subprojects related to this work are:

A.  Determining the effect of shear stress at hypothermic temperatures on endothelial cells in the isolated livers. Our group is able to machine perfused a liver on a temperature controlled microscope stage and image the sinusoidal structure of the liver with intravital microscopy.

B.  Understand the effect of shear stress on endothelial cells in a microshear chamber at hypothermic temperatures. Using primary cultures of liver endothelial cells we are using video microscopy to assess endothelial cell shape as a function of shear stress.

C.  Investigate the potential of reclaiming non-heart-beating donor livers with machine perfusion. Donors that experience cardiac arrest prior to removal of their organs experience periods of warm ischemia which make the organs unusable. Our group has shown that machine perfusion has the potential to reclaim and preserve for short periods these organs for transplant.

D.  Investigate the potential in preserving fatty livers by HMP.

E.  Collaboration with Carolinas Medical Center Transplantation Group and Organ Recovery Systems to scale-up HMP livers for transplants.

 

Bioinstrumentation:

Our group is finding that we need to build instruments for our research purposes and that these devices may have broader applications. We will continue to build such devices for diagnostics and imaging purposes.

 

Selected Publications:Pegg, D.E., Rubinsky, B., Diaper, M., Lee, C.Y. (1986) Analysis of the introduction and removal of glycerol in rabbit kidney using a Krogh cylinder model, Cryobiology, 23, 150-160.

Rubinsky, B., Lee, C.Y., Bastacky J., and Hayes, T.L., (1987), The mechanism of freezing in biological tissue:  the liver, Cryo-Letters, 8: 370-381.

C.Y. Lee and B. Rubinsky, “Multidimensional Analysis of Momentum and Mass Transfer in the Hepatic Acinus,” in Computational Methods in Bioengineering, R.L. Spilker and B.R. Simon Eds., ASME Press, NY, NY, 1988, pp. 267-280 (Invited).

Lee, C.Y. and Rubinsky, B., (1989) A multi-dimensional model of momentum and mass transfer in the liver, Int. J. Heat and Mass Transfer 32: 2421-2434

Lee, C.Y., Rubinsky, B., and Fletcher, G.L., (1992) Hypothermic preservation of whole mammalian organs with “antifreeze” proteins, Cryo-Letter, 8: 190-198.

Rubinsky B., Arav A., Hong J.S., and Lee C.Y., (1994) Freezing of mammalian livers with glycerol and antifreeze proteins, Biochemical and Biophysical Research Communications, 200 (2): 732-41.

Lee, C.Y., Matsumoto-Pon, J., and Widdicombe, J.H., (1997) Cultured lung epithelium: cellular model for lung preservation, Cryobiology 35: 209-218.

Ishine, N., Rubinsky, B., and Lee, C.Y., (1999) A histological analysis of liver injury in freezing storage, Cryobiology, 39 (4): 271-277.

Ishine, N., Rubinsky, B., and Lee, C.Y., (2000) Transplantation of mammalian livers after freezing: vascular damage and functional recovery, Cryobiology, 40: 84-89.

Lee, C.Y., Zhang, J.X., deSilva, H., Coger, R.N., and Clemens, M.G., (2000) Heterogeneous flow patterns during hypothermic machine perfusion preservation of livers Transplantation 70: 1797-1802.

Purohit, S., Nelson, J., Zhang, J.X., Clemens, M.G., and Lee, C.Y. (2001) Flow dynamics during machine perfusion preservation of livers for transplantation, Proceedings of the 2001 International Mechanical Engineering Congress and Exposition.

Venkatapathi, M., and Lee, C.Y., (2001) Portable fluorometry for kinetics application, Proceedings of the 2001 International Mechanical Engineering Congress and Exposition.

Lee, C.Y., Zhang, J.X., Jones, J.W. Jr., Southard, J.H., Clemens, M.G., (2002) Functional recovery of preserved livers following warm ischemia:  Improvements by machine perfusion preservation, Transplantation, 74(7): 944-951.

Slater, J.H., Jain, S, Coger, R.N., and Lee, C.Y., (2002) The effect of shear stress on endothelial cells at hypothermic temperature, Proceedings of the 2002 International Mechanical Engineering Congress and Exposition.

Lee, C. Y., Jain, S, Duncan, H. M., Zhang, J.X., Jones, J.W.Jr, Southard, J.H., Clemens, M.G. (2003) Survival transplantation of non-heart-beating donor rat livers following hypothermic machine perfusion preservation, Transplantation 76 (10) pp. 1432-36.

Hongzhi Xu, Charles Y. Lee,, Mark G. Clemens, Jian X. Zhang, (2004) Prolonged Hypothermic Machine Perfusion Preserves Hepatocellular Function but Potentiates Endothelial Cell Dysfunction in Rat Livers, Transplantation 77:11, pp 1676-82.

Jain, S., Purohit, S., Xu, H., J.X. Zhang, Clemens, M.G., and Lee, C.Y. (2004) Ex vivo study of flow dynamics and endothelial cell structure during extended hypothermic machine perfusion preservation of livers, Cryobiology 48, pp. 322-332.

Xu, H, Zhang, J.X., Jones, J.W.Jr, Southard, J.H., Clemens, M.G., Lee, C.Y., Hypothermic Machine Perfusion of Rat Livers Preserves Endothelial Cell Function, presented at the 20th International Congress of the Transplant Society and to appear in Transplantation Proceedings.

S.Jain, C.Y.Lee, S.Baicu, H.Duncan, H.Xu, J.W.Jones Jr, M.G.Clemens, J.Brassil, M.J.Taylor, K.G.M.Brockbank, (2004) Hepatic Function in Hypothermically Stored Porcine Livers: Comparison of Hypothermic Machine Perfusion vs. Cold Storage, presented at the 20th International Congress of the Transplant Society and to appear in Transplantation Proceedings.

Funding:

The Whitaker Foundation Biomedical Engineering Research Grant

National Institutes of Health’s Bioengineering Research Partnership Award

 

 

  • Alerts
  • Jobs
  • Make a Gift
  • Maps / Directions
  • Accessibility IconAccessibility
Follow UNC Charlotte
Facebook Blogger Twitter Flickr YouTube
The University of North Carolina at Charlotte 9201 University City Blvd., Charlotte, NC 28223-0001 · 704-687-UNCC (8622) © 2014 UNC Charlotte | All Rights Reserved | Terms of Use | Policy Statements | Contact Us
Skip to toolbar
  • Log In