1-People

PI: Dr. Shan Yan

Professor, University of North Carolina at Charlotte, Charlotte, NC, 2019-present

Associate Professor, University of North Carolina at Charlotte, Charlotte, NC

Assistant Professor, University of North Carolina at Charlotte, Charlotte, NC

Research Associate, Harvard University, Cambridge, MA

Postdoctoral Fellow, Harvard University, Cambridge, MA

Ph.D., Fudan University, Shanghai, China

B.S. & M.S., Shandong University, Jinan, China

Lab Members

Research Associate

Jia Li, PhD

Haichao Zhao, PhD

 

Postdoctoral Fellow

 

Graduate Students

Garrett Driscoll

Joshua Matos

Anne McMahon

Kelly McGhee

 

Undergraduate Students

Amogh Bandekar: Levine Scholar

Brianna Bush: Charlotte Research Scholar

Kyleigh Payne: OUR Scholar

 

 

 

 

Lab photos

Summer of 2023

Fall of 2020

labpic-fall2016

Fall of 2016

 lab-10-31-2014

Fall of 2014

summer2013-2

Summer of 2013

Winter of 2012

Summer 2012

 

 

 

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Former Lab Members

Research Faculty

Yunfeng Lin, PhD (2022-2023): Research Assistant Professor

 

Postdoctoral Fellow

Liping Bai, PhD (2012-2014): now Professor @Peking Union Medical College, China

Yunfeng Lin, PhD (2015-2022)

Jia Li, PhD (2018-2023)

Graduate Students

(8) Md. Akram Hossain (2016-2021): PhD. Graduate School Summer Fellowship (2019 & 2020)

Publications:

(a) Hossain MA, Lin Y#, Yan S*. 2018. SSB end resection in genome stability: mechanism and regulation by APE2. International Journal of Molecular Sciences. 19 (8): 2389.  (PMCID: PMC6122073; PMID:30110897) DOI: https://doi.org/10.3390/ijms19082389

(b) Lin Y§,#, Raj J§,, Li J#, Ha A, Hossain MA, Richardson C, Mukherjee P, Yan S*. 2020. APE1 senses DNA single-strand breaks for repair and signaling. Nucleic Acids Research. 48(4):1925-1940 (PMCID: PMC7038996; PMID: 31828326; Epub ahead of print) DOI: https://doi.org/10.1093/nar/gkz1175

(c) Lin Y§,#, Bai L§,#, Cupello S, Hossain MA, Deem B, McLeod M, Raj J, Yan S*. 2018. APE2 promotes DNA damage response pathway from a single-strand break. Nucleic Acids Research. 46 (5): 2479-2494. (PMCID: PMC5861430; PMID: 29361157) DOI: https://doi.org/10.1093/nar/gky020

(7) Anh Ha (2018-2020): M.S.,

Publications:

(a) Ha A, Lin Y#, and Yan S*. 2020. A non-canonical role for the DNA glycosylase NEIL3 in suppressing APE1 endonuclease-mediated ssDNA damage. Journal of Biological Chemistry (online ahead of print August 14, 2020). DOI: https://doi.org/10.1074/jbc.RA120.014228

(b) Lin Y§,#, Raj J§,, Li J#, Ha A, Hossain MA, Richardson C, Mukherjee P, Yan S*. 2020. APE1 senses DNA single-strand breaks for repair and signaling. Nucleic Acids Research. 48(4):1925-1940 (PMCID: PMC7038996; PMID: 31828326; Epub ahead of print) DOI: https://doi.org/10.1093/nar/gkz1175

(c)  Lin Y#, Ha Anh, Yan S*. 2019. Methods for studying DNA single-strand break repair and signaling in Xenopus egg extracts. Methods in Molecular Biology: DNA Repair Methods and Protocols.  1999: 161-172. (PMCID: PMC6550457; PMID: 31127575) (Balakrishnan and Stewart eds). DOI: https://doi.org/10.1007/978-1-4939-9500-4_9

(6) Steven Cupello (2014-2019): PhD. Graduate School Summer Fellowship (2018)

Publications:

(a) Cupello S, Lin Y#, Yan S*. 2019. Distinct roles of XRCC1 in genome integrity in Xenopus egg extracts. Biochemical Journal. 476(24):3791-3804. (PMCID: PMC6959006; PMID: 31808793) DOI: https://doi.org/10.1042/BCJ20190798

(b) Lin Y§,#, Bai L§,#, Cupello S, Hossain MA, Deem B, McLeod M, Raj J, Yan S*. 2018. APE2 promotes DNA damage response pathway from a single-strand break. Nucleic Acids Research. 46 (5): 2479-2494. (PMCID: PMC5861430; PMID: 29361157) DOI: https://doi.org/10.1093/nar/gky020

(c) Cupello S, Richardson C, and Yan S*. 2016. Cell-free Xenopus egg extracts for studying DNA damage response pathways. International Journal of Developmental Biology. 60 (7-8-9): 229-236. (PMCID: PMC5071109; PMID: 27160070) DOI: http://dx.doi.org/10.1387/ijdb.160113sy

(5) Melissa McLeod (2014-2016): M.S.. The 2018 Graduate School Outstanding M.S. Thesis Award

Publications:

(a) DeStephanis D§,, McLeod M§,, Yan S*. 2015. REV1 is important for the ATR-Chk1 DNA damage response pathway in Xenopus egg extracts. Biochemical and Biophysical Research Communications. 460 (3): 609-615.(PMCID: PMC4426025; PMID: 25800873) DOI: http://dx.doi.org/10.1016/j.bbrc.2015.03.077

(b) Lin Y§,#, Bai L§,#, Cupello S, Hossain MA, Deem B, McLeod M, Raj J, Yan S*. 2018. APE2 promotes DNA damage response pathway from a single-strand break. Nucleic Acids Research. 46 (5): 2479-2494. (PMCID: PMC5861430; PMID: 29361157) DOI: https://doi.org/10.1093/nar/gky020

(4) Bradley Deem (2014-2015): M.S. transfer to non-thesis track

Publications:

(a) Lin Y§,#, Bai L§,#, Cupello S, Hossain MA, Deem B, McLeod M, Raj J, Yan S*. 2018. APE2 promotes DNA damage response pathway from a single-strand break. Nucleic Acids Research. 46 (5): 2479-2494. (PMCID: PMC5861430; PMID: 29361157) DOI: https://doi.org/10.1093/nar/gky020

(3) Zachary Berman  (2013-2015): M.S. student.

Publications:

(a) Wallace BD§, Berman Z§, ¶,, Mueller GA, Lin Y#, Chang T, Andres SN, Wojtaszek JL, DeRose EF, Appel CD, London RE, Yan S*, Williams RS*. 2017.  APE2 Zf-GRF facilitates 3′-5′ resection of DNA damage following oxidative stress. Proceedings of the National Academy of Sciences of the United States of America. 114 (2): 304-309. (PMCID: PMC5240719; PMID: 28028224) DOI: 10.1073/pnas.1610011114

(b) Yan S*, Sorrell M, Berman Z. 2014. Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress. Cellular and Molecular Life Sciences. 71 (20): 3951-3967. (PMCID: PMC4176976; PMID: 24947324) DOI: http://dx.doi.org/10.1007/s00018-014-1666-4

(2) Jeremy Willis (2011-2013): M.S.

Publications:

(a) Willis J§,, Patel Y§,, Lentz B,  Yan S*. 2013. APE2 is required for ATR-Chk1 checkpoint activation in response to oxidative stress. Proceedings of the National Academy of Sciences of the United States of America. 110 (26): 10592-10597. (PMCID: PMC3696815; PMID: 23754435).  DOI: http://dx.doi.org/10.1073/pnas.1301445110

(b) Yan S*, Willis J. 2013. WD40-repeat protein WDR18 collaborates with TopBP1 to facilitate DNA damage checkpoint signaling. Biochemical and Biophysical Research Communications. 431 (2): 466-471. (PMCID: PMC3577970; PMID: 23333389). DOI: http://dx.doi.org/10.1016/j.bbrc.2012.12.144

(c) Willis J§,, DeStephanis D§,, Patel Y, Gowda V, Yan S*.2012. Study of the DNA damage checkpoint using Xenopus egg extracts. Journal of Visualized Experiments. (69): e4449. (PMCID: PMC3514051; PMID: 23149695). DOI: http://dx.doi.org/10.3791/4449

(1) Darla DeStephanis (2010-2013): M.S.

Publications:

(a) DeStephanis D§,, McLeod M§,, Yan S*. 2015. REV1 is important for the ATR-Chk1 DNA damage response pathway in Xenopus egg extracts. Biochemical and Biophysical Research Communications. 460 (3): 609-615.(PMCID: PMC4426025; PMID: 25800873) DOI: http://dx.doi.org/10.1016/j.bbrc.2015.03.077

(b) Willis J§,, DeStephanis D§,, Patel Y, Gowda V, Yan S*.2012. Study of the DNA damage checkpoint using Xenopus egg extracts. Journal of Visualized Experiments. (69): e4449. (PMCID: PMC3514051; PMID: 23149695). DOI: http://dx.doi.org/10.3791/4449

Undergraduate Students

(21) Naya Kayali (2020-2021):

(20) Amit Thota (2019-2020): Charlotte Research Scholar

(19) Adam Dignan (2019): Charlotte Research Scholar

(18) Emily Floyd (2018-2019):

(17) Katherine Jensen (2017-2018): Charlotte Research Scholar, ANSWER Scholar

(16) Anh Ha (2016-2018): NSF REU Fellow

(15) Mary Tess Overton (2015-2018): Barry Goldwater Scholar, NSF REU Fellow

(14) Victoria Fitts (2016): NSF REU Fellow, undergraduate student from Santa Fe College

(13) Jude Raj (2013-2016): NSF REU Fellow, Honors student

(12) Dafe Omerhi (2015-2016):

(11) Krystal Carmichael (2014-2015): Charlotte Research Scholar, Honors student

(10) Harleen Kaur (2013-2014):

(9)  Peyman “Alex” Gharavizadeh (2014):

(8)  Jagat Patel (2013):

(7)  Alexa R. Campbell (2013): undergraduate student from UNC-Chapel Hill

(6)  James Hooper (2012):

(5)  Mallorie Hartmann (2012-2013):

(4)  Mohammad Ahmad (2012):

(3)  Yogin Patel (2011-2012): Honors student

(2)  Barry Lentz (2011): Honors student

(1)  Vrushab Gowda (2010-2012): Levine Scholar

Post-Bac student

(1) John Curtis Dukes (2011):

High School Students

Vijay Rachakonda