1-People

PI: Dr. Shan Yan Professor, University of North Carolina at Charlotte, Charlotte, NC, 2019-present Associate Professor, University of North Carolina at Charlotte, Charlotte, NC Assistant Professor, University of North Carolina at Charlotte, Charlotte, NC Research Associate, Harvard University, Cambridge, MA Postdoctoral Fellow, Harvard University, Cambridge, MA Ph.D., Fudan University, Shanghai, China B.S. & M.S., Shandong University, Jinan, China

Lab Members Research Associate Jia Li, PhD Haichao Zhao, PhD   Postdoctoral Fellow   Graduate Students Garrett Driscoll Joshua Matos Anne McMahon Kelly McGhee   Undergraduate Students Jade Lyttle: OUR Scholar Anthony Malone: OUR Scholar

Lab photos

Summer of 2023

Fall of 2020

Fall of 2016

 

Fall of 2014

Summer of 2013

Winter of 2012

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Former Lab Members

Research Faculty

Yunfeng Lin, PhD (2022-2023): Research Assistant Professor

 

Postdoctoral Fellow

Liping Bai, PhD (2012-2014): now Professor @Peking Union Medical College, China

Yunfeng Lin, PhD (2015-2022)

Jia Li, PhD (2018-2023)

 

Graduate Students

(8) Md. Akram Hossain (2016-2021): PhD. Graduate School Summer Fellowship (2019 & 2020)

Publications:

(a) Hossain MA^¶, Lin Y^#, Yan S*. 2018. SSB end resection in genome stability: mechanism and regulation by APE2. International Journal of Molecular Sciences. 19 (8): 2389.  (PMCID: PMC6122073; PMID:30110897) DOI: https://doi.org/10.3390/ijms19082389

(b) Lin Y^§,#, Raj J^§,‡, Li J^#, Ha A^¶, Hossain MA^¶, Richardson C, Mukherjee P, Yan S*. 2020. APE1 senses DNA single-strand breaks for repair and signaling. Nucleic Acids Research. 48(4):1925-1940 (PMCID: PMC7038996; PMID: 31828326; Epub ahead of print) DOI: https://doi.org/10.1093/nar/gkz1175

(c) Lin Y^§,#, Bai L^§,#, Cupello S^¶, Hossain MA^¶, Deem B^¶, McLeod M^¶, Raj J^‡, Yan S*. 2018. APE2 promotes DNA damage response pathway from a single-strand break. Nucleic Acids Research. 46 (5): 2479-2494. (PMCID: PMC5861430; PMID: 29361157) DOI: https://doi.org/10.1093/nar/gky020

(7) Anh Ha (2018-2020): M.S.,

Publications:

(a) Ha A^¶, Lin Y^#, and Yan S*. 2020. A non-canonical role for the DNA glycosylase NEIL3 in suppressing APE1 endonuclease-mediated ssDNA damage. Journal of Biological Chemistry (online ahead of print August 14, 2020). DOI: https://doi.org/10.1074/jbc.RA120.014228

(b) Lin Y^§,#, Raj J^§,‡, Li J^#, Ha A^¶, Hossain MA^¶, Richardson C, Mukherjee P, Yan S*. 2020. APE1 senses DNA single-strand breaks for repair and signaling. Nucleic Acids Research. 48(4):1925-1940 (PMCID: PMC7038996; PMID: 31828326; Epub ahead of print) DOI: https://doi.org/10.1093/nar/gkz1175

(c)  Lin Y^#, Ha Anh^¶‡, Yan S*. 2019. Methods for studying DNA single-strand break repair and signaling in Xenopus egg extracts. Methods in Molecular Biology: DNA Repair Methods and Protocols.  1999: 161-172. (PMCID: PMC6550457; PMID: 31127575) (Balakrishnan and Stewart eds). DOI: https://doi.org/10.1007/978-1-4939-9500-4_9

(6) Steven Cupello (2014-2019): PhD. Graduate School Summer Fellowship (2018)

Publications:

(a) Cupello S^¶, Lin Y^#, Yan S*. 2019. Distinct roles of XRCC1 in genome integrity in Xenopus egg extracts. Biochemical Journal. 476(24):3791-3804. (PMCID: PMC6959006; PMID: 31808793) DOI: https://doi.org/10.1042/BCJ20190798

(b) Lin Y^§,#, Bai L^§,#, Cupello S^¶, Hossain MA^¶, Deem B^¶, McLeod M^¶, Raj J^‡, Yan S*. 2018. APE2 promotes DNA damage response pathway from a single-strand break. Nucleic Acids Research. 46 (5): 2479-2494. (PMCID: PMC5861430; PMID: 29361157) DOI: https://doi.org/10.1093/nar/gky020

(c) Cupello S^¶, Richardson C, and Yan S^*. 2016. Cell-free Xenopus egg extracts for studying DNA damage response pathways. International Journal of Developmental Biology. 60 (7-8-9): 229-236. (PMCID: PMC5071109; PMID: 27160070) DOI: http://dx.doi.org/10.1387/ijdb.160113sy

(5) Melissa McLeod (2014-2016): M.S.. The 2018 Graduate School Outstanding M.S. Thesis Award

Publications:

(a) DeStephanis D^§,¶, McLeod M^§,¶, Yan S^*. 2015. REV1 is important for the ATR-Chk1 DNA damage response pathway in Xenopus egg extracts. Biochemical and Biophysical Research Communications. 460 (3): 609-615.(PMCID: PMC4426025; PMID: 25800873) DOI: http://dx.doi.org/10.1016/j.bbrc.2015.03.077

(b) Lin Y^§,#, Bai L^§,#, Cupello S^¶, Hossain MA^¶, Deem B^¶, McLeod M^¶, Raj J^‡, Yan S*. 2018. APE2 promotes DNA damage response pathway from a single-strand break. Nucleic Acids Research. 46 (5): 2479-2494. (PMCID: PMC5861430; PMID: 29361157) DOI: https://doi.org/10.1093/nar/gky020

(4) Bradley Deem (2014-2015): M.S. transfer to non-thesis track

Publications:

(a) Lin Y^§,#, Bai L^§,#, Cupello S^¶, Hossain MA^¶, Deem B^¶, McLeod M^¶, Raj J^‡, Yan S*. 2018. APE2 promotes DNA damage response pathway from a single-strand break. Nucleic Acids Research. 46 (5): 2479-2494. (PMCID: PMC5861430; PMID: 29361157) DOI: https://doi.org/10.1093/nar/gky020

(3) Zachary Berman  (2013-2015): M.S. student.

Publications:

(a) Wallace BD^§, Berman Z^{§, ¶},, Mueller GA, Lin Y^#, Chang T, Andres SN, Wojtaszek JL, DeRose EF, Appel CD, London RE, Yan S^*, Williams RS^*. 2017.  APE2 Zf-GRF facilitates 3′-5′ resection of DNA damage following oxidative stress. Proceedings of the National Academy of Sciences of the United States of America. 114 (2): 304-309. (PMCID: PMC5240719; PMID: 28028224) DOI: 10.1073/pnas.1610011114

(b) Yan S^*, Sorrell M, Berman Z^¶. 2014. Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress. Cellular and Molecular Life Sciences. 71 (20): 3951-3967. (PMCID: PMC4176976; PMID: 24947324) DOI: http://dx.doi.org/10.1007/s00018-014-1666-4

(2) Jeremy Willis (2011-2013): M.S.

Publications:

(a) Willis J^§,¶, Patel Y^§,‡, Lentz B ^‡,  Yan S^*. 2013. APE2 is required for ATR-Chk1 checkpoint activation in response to oxidative stress. Proceedings of the National Academy of Sciences of the United States of America. 110 (26): 10592-10597. (PMCID: PMC3696815; PMID: 23754435).  DOI: http://dx.doi.org/10.1073/pnas.1301445110

(b) Yan S^*, Willis J^¶. 2013. WD40-repeat protein WDR18 collaborates with TopBP1 to facilitate DNA damage checkpoint signaling. Biochemical and Biophysical Research Communications. 431 (2): 466-471. (PMCID: PMC3577970; PMID: 23333389). DOI: http://dx.doi.org/10.1016/j.bbrc.2012.12.144

(c) Willis J^§,¶, DeStephanis D^§,¶, Patel Y^‡, Gowda V^‡, Yan S^*.2012. Study of the DNA damage checkpoint using Xenopus egg extracts. Journal of Visualized Experiments. (69): e4449. (PMCID: PMC3514051; PMID: 23149695). DOI: http://dx.doi.org/10.3791/4449

(1) Darla DeStephanis (2010-2013): M.S.

Publications:

(a) DeStephanis D^§,¶, McLeod M^§,¶, Yan S^*. 2015. REV1 is important for the ATR-Chk1 DNA damage response pathway in Xenopus egg extracts. Biochemical and Biophysical Research Communications. 460 (3): 609-615.(PMCID: PMC4426025; PMID: 25800873) DOI: http://dx.doi.org/10.1016/j.bbrc.2015.03.077

(b) Willis J^§,¶, DeStephanis D^§,¶, Patel Y^‡, Gowda V^‡, Yan S^*.2012. Study of the DNA damage checkpoint using Xenopus egg extracts. Journal of Visualized Experiments. (69): e4449. (PMCID: PMC3514051; PMID: 23149695). DOI: http://dx.doi.org/10.3791/4449

 

 

Undergraduate Students

(4)  Mohammad Ahmad (2012):

(2)  Barry Lentz (2011): Honors student

(1)  Vrushab Gowda (2010-2012): Levine Scholar

Post-Bac student

(1) John Curtis Dukes (2011):

High School Students

Vijay Rachakonda